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KMID : 0363620090300040129
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Journal of Korean Oriental Medicine
2009 Volume.30 No. 4 p.129 ~ p.141
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The Anticancer Effects and Drug Metabolic Enzyme Change by Intraperitoneal Injection of Agrimonia Pilosa Ledeb
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Chio Jung-Won
Park Jong-Hyung
Jung Hee
Lee Ju-Ah
Ko Seong-Gyu
Jang Bo-Hyoung
Go Ho-Yeon
Jeon Chan-Yong
Kim Ji-Hye
Choi You-Kyung
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Abstract
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Objective: This study was to investigate the anti-tumor effect, safety, safety, mechanism and metabolizing enzyme of Agrimonia pilosa LEDEB (APL) in female C57B/L mouse tumor (in vivo).
Method: First, to evaluate the antitumor activity of APL, we divided the mice into four groups: normal, control, APL50 (50mg/kg), and APL100 (100mg/kg). LLC-obtained American Type Culture Collection was used. LLC had been inoculated to induce tumors. To measure the anti-tumor effect of APL, we calibrated tumor size and weight. To analyze the mechanism of anti-tumor in APL, we used western blotting and to observe metabolizing enzyme in APL we used to real-time PCR.
Result: APL50 and APL100 significantly inhibited tumor growth from 12 days after medicine injected. APL did not induce caspase-dependent apoptosis in LLC-bearing mouse tumor. In APL100, it decreased 41% and 71% in CYP2D22 and CYP3A11, respectively.
Conclusion: These results suggest that APL has some anti-tumor effects in female C57B/L mouse tumor. APL should be used carefully with other drugs related with CYP2D22 and CYP3A11.
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KEYWORD
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APL, anti-tumor, PCR, western blot, LLC
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